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What is SHOTGUN SEQUENCING? What does SHOTGUN SEQUENCING mean? SHOTGUN SEQUENCING meaningWhat is SHOTGUN SEQUENCING? What does SHOTGUN SEQUENCING mean? SHOTGUN SEQUENCING meaning What is SHOTGUN SEQUENCING? What does SHOTGUN SEQUENCING mean? SHOTGUN SEQUENCING meaning


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What is SHOTGUN SEQUENCING? What does SHOTGUN SEQUENCING mean? SHOTGUN SEQUENCING meaning Source Wikipedia org article, adapted under s creativecommons org licenses by-sa 3 0 license In genetics, shotgun sequencing, also known as shotgun cloning, is a method used for sequencing long DNA strands It is named by analogy with the rapidly expanding, quasi-random firing pattern of a shotgun The chain termination method of DNA sequencing (or "Sanger sequencing" for its developer Frederick Sanger) can only be used for fairly short strands of 100 to 1000 base pairs Longer sequences are subdivided into smaller fragments that can be sequenced separately, and subsequently they are re-assembled to give the overall sequence Two principal methods are used for this primer walking (or "chromosome walking") which progresses through the entire strand piece by piece, and shotgun sequencing, which is a faster but more complex process that uses random fragments In shotgun sequencing, DNA is broken up randomly into numerous small segments, which are sequenced using the chain termination method to obtain reads Multiple overlapping reads for the target DNA are obtained by performing several rounds of this fragmentation and sequencing Computer programs then use the overlapping ends of different reads to assemble them into a continuous sequence Shotgun sequencing was one of the precursor technologies that was responsible for enabling full genome sequencing

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Shotgun sequencing method explained

This lecture explains about the shotgun sequencing method This DNA sequencing method is used to clone and sequence large genome content during the human genome project Shotgun DNA sequencing is the best approach to sequence big DNA content after the mechanical shearing The fragmented DNA is then sequenced with Sanger method or dideoxy method or any other next generation sequencing methods of the DNA For more information, log on to- www shomusbiology com Get Shomu s Biology DVD set here- www shomusbiology com dvd-store Download the study materials here- shomusbiology com bio-materials html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube All these tutorials are brought to you for free Please subscribe to our channel so that we can grow together You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www shomusbiology com dvd-store Shomu’s Biology assignment services – www shomusbiology com assignment -help Join Online coaching for CSIR NET exam – www shomusbiology com net-coaching We are social Find us on different sites here- Our Website – www shomusbiology com Facebook page- s www facebook com ShomusBiology Twitter - s twitter com shomusbiology SlideShare- www slideshare net shomusbiology Google plus- s plus google com 113648584982732129198 LinkedIn - s www linkedin com in suman-bhattacharjee-2a051661 Youtube- s www youtube com user TheFunsuman Thank you for watching the video lecture on the shotgun sequencing of the

When do I use Sanger Sequencing vs NGS? Seq It Out #7

Download the free Sanger sequencing handbook at www thermofisher com sangerhandbook Submit your question at www thermofisher com ask Planning a sequencing experiment? I am sure you have a lot of questions like, Which is the right sequencing method for your application? Sanger or next generation sequencing? Let’s take a look at both in this episode of Seq It Out Before the 1990s, sequencing was done the “old fashioned” way; using gels and manually calling bases Then, Sanger Sequencing by capillary electrophoresis was introduced This let labs sequence long DNA fragments with automated base calls As the technology evolved, Next Generation Sequencing, or NGS, transformed the way that sequencing was performed Let’s take a look at our lab book NGS is similar to Sanger sequencing in that you sequence DNA fragments But in NGS, it’s massively parallel This allows millions of fragments to be sequenced in a single run versus sanger sequencing which only produces one forward and reverse read Researchers today have many choices when deciding which sequencing technology to use for their clinical research Sanger sequencing with 99 99% accuracy is the “gold standard” for clinical research sequencing However, newer NGS technologies are also becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample But which one should you use? Sanger or NGS? Here is what you have to consider With its ability for long reads and gold-standard accuracy, Sanger sequencing is perfect for • Sequencing single genes • Sequencing 1-100 amplicon targets at the lowest cost • Sequencing up to 96 samples at a time without barcoding • Microbial Identification • Fragment analysis, high throughput genotyping using, for example, SNaPshot • Microsatellite or STR analysis • NGS confirmation Next generation sequencing offers fast turnaround time and takes only about 4 hours to complete a run NGS is perfect for • Interrogating greater than 100 genes at a time cost effectively • Finding novel variants by expanding the number of targets sequenced in a single run • Sequencing samples that have low input amounts of starting material, using, for example, Ion AmpliSeq library preparation, which requires as little as 10 ng of input DNA • Sequencing microbial genomes for pathogen subtyping to enable research of critical outbreak situations With Applied Biosystems gold standard Sanger sequencing and simple, scalable Ion Torrent™ next-generation sequencing, Thermo Fisher Scientific has the right sequencing platform for every research application I hope this video was helpful on finding the right sequencing tool for your application, and I am sure you’ll have more questions Submit your question at www thermofisher com ask and subscribe to our channel to see more videos like this And remember, when in doubt, just Seq I

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