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It is a video caused by the uploader with such an awesome exertion and have the craving and commitment to benefit you or inform you about Shotgun sequencing (hierarchial shotgun sequencing). Moreover would like to add to your enthusiasm under Enlightenment and I trust the two of us delighted you. There are 39664 watchers who left their remarks.
For more information, log on to- shomusbiology weebly com Download the study materials here- shomusbiology weebly com bio-materials html Although shotgun sequencing can in theory be applied to a genome of any size, its direct application to the sequencing of large genomes (for instance, the Human Genome) was limited until the late 1990s, when technological advances made practical the handling of the vast quantities of complex data involved in the process [9] Historically, full-genome shotgun sequencing was believed to be limited by both the sheer size of large genomes and by the complexity added by the high percentage of repetitive DNA (greater than 50% for the human genome) present in large genomes [10] It was not widely accepted that a full-genome shotgun sequence of a large genome would provide reliable data For these reasons, other strategies that lowered the computational load of sequence assembly had to be utilized before shotgun sequencing was performed [10] In hierarchical sequencing, also known as top-down sequencing, a low-resolution physical map of the genome is made prior to actual sequencing From this map, a minimal number of fragments that cover the entire chromosome are selected for sequencing [11] In this way, the minimum amount of high-throughput sequencing and assembly is required The amplified genome is first sheared into larger pieces (50-200kb) and cloned into a bacterial host using BACs or PACs Because multiple genome copies have been sheared at random, the fragments contained in these clones have different ends, and with enough coverage (see section above) finding a scaffold of BAC contigs that covers the entire genome is theoretically possible This scaffold is called a tiling path Source of the article published in description is Wikipedia I am sharing their material Copyright by original content developers Link- en wikipedia org wiki Main_Page

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Shotgun sequencing method explained

This lecture explains about the shotgun sequencing method This DNA sequencing method is used to clone and sequence large genome content during the human genome project Shotgun DNA sequencing is the best approach to sequence big DNA content after the mechanical shearing The fragmented DNA is then sequenced with Sanger method or dideoxy method or any other next generation sequencing methods of the DNA For more information, log on to- www shomusbiology com Get Shomu s Biology DVD set here- www shomusbiology com dvd-store Download the study materials here- shomusbiology com bio-materials html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube All these tutorials are brought to you for free Please subscribe to our channel so that we can grow together You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www shomusbiology com dvd-store Shomu’s Biology assignment services – www shomusbiology com assignment -help Join Online coaching for CSIR NET exam – www shomusbiology com net-coaching We are social Find us on different sites here- Our Website – www shomusbiology com Facebook page- s www facebook com ShomusBiology Twitter - s twitter com shomusbiology SlideShare- www slideshare net shomusbiology Google plus- s plus google com 113648584982732129198 LinkedIn - s www linkedin com in suman-bhattacharjee-2a051661 Youtube- s www youtube com user TheFunsuman Thank you for watching the video lecture on the shotgun sequencing of the

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