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Whole genome shotgun sequencingWhole genome shotgun sequencing Whole genome shotgun sequencing


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It is a video caused by the uploader with such an awesome exertion and have the craving and commitment to benefit you or inform you about Whole genome shotgun sequencing. Moreover would like to add to your enthusiasm under Enlightenment and I trust the two of us delighted you. There are 46517 watchers who left their remarks.
For more information, log on to- shomusbiology weebly com Download the study materials here- shomusbiology weebly com bio-materials html Whole genome shotgun sequencing for small (4000 to 7000 basepair) genomes was already in use in 1979 [1] Broader application benefited from pairwise end sequencing, known colloquially as double-barrel shotgun sequencing As sequencing projects began to take on longer and more complicated DNAs, multiple groups began to realize that useful information could be obtained by sequencing both ends of a fragment of DNA Although sequencing both ends of the same fragment and keeping track of the paired data was more cumbersome than sequencing a single end of two distinct fragments, the knowledge that the two sequences were oriented in opposite directions and were about the length of a fragment apart from each other was valuable in reconstructing the sequence of the original target fragment The first published description of the use of paired ends was in 1990[3] as part of the sequencing of the human HGPRT locus, although the use of paired ends was limited to closing gaps after the application of a traditional shotgun sequencing approach The first theoretical description of a pure pairwise end sequencing strategy, assuming fragments of constant length, was in 1991 [4] At the time, there was community consensus that the optimal fragment length for pairwise end sequencing would be three times the sequence read length In 1995 Roach et al [5] introduced the innovation of using fragments of varying sizes, and demonstrated that a pure pairwise end-sequencing strategy would be possible on large targets The strategy was subsequently adopted by The Institute for Genomic Research (TIGR) to sequence the genome of the bacterium Haemophilus influenzae in 1995,[6] and then by Celera Genomics to sequence the Drosophila melanogaster (fruit fly) genome in 2000,[7] and subsequently the human genome To apply the strategy, high-molecular-weight DNA is sheared into random fragments, size-selected (usually 2, 10, 50, and 150 kb), and cloned into an appropriate vector The clones are then sequenced from both ends using the chain termination method yielding two short sequences Each sequence is called an end-read or read and two reads from the same clone are referred to as mate pairs Since the chain termination method usually can only produce reads between 500 and 1000 bases long, in all but the smallest clones, mate pairs will rarely overlap Source of the article published in description is Wikipedia I am sharing their material Copyright by original content developers Link- en wikipedia org wiki Main_Page

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Shotgun sequencing method explained

This lecture explains about the shotgun sequencing method This DNA sequencing method is used to clone and sequence large genome content during the human genome project Shotgun DNA sequencing is the best approach to sequence big DNA content after the mechanical shearing The fragmented DNA is then sequenced with Sanger method or dideoxy method or any other next generation sequencing methods of the DNA For more information, log on to- www shomusbiology com Get Shomu s Biology DVD set here- www shomusbiology com dvd-store Download the study materials here- shomusbiology com bio-materials html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube All these tutorials are brought to you for free Please subscribe to our channel so that we can grow together You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www shomusbiology com dvd-store Shomu’s Biology assignment services – www shomusbiology com assignment -help Join Online coaching for CSIR NET exam – www shomusbiology com net-coaching We are social Find us on different sites here- Our Website – www shomusbiology com Facebook page- s www facebook com ShomusBiology Twitter - s twitter com shomusbiology SlideShare- www slideshare net shomusbiology Google plus- s plus google com 113648584982732129198 LinkedIn - s www linkedin com in suman-bhattacharjee-2a051661 Youtube- s www youtube com user TheFunsuman Thank you for watching the video lecture on the shotgun sequencing of the

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